Institute of agroecology and environmental management of National Academy of Agrarian Sciences (IAEM), Ukraine, Research at IAEM is aimed to increase the role of the environmental component of agriculture in Ukraine. As a result of the use of mineral nitrogen fertilizers, industry production and the number of farm animals, the problem of eutrophication of water sources is particularly acute. This indicates the loss of nitrogen throughout the all nitrogen cycle. The contribution focuses on the East Europe regional demonstration (Dniester, Prut and Lower Danube). The activity aims to demonstrate how a cross cutting approach that joins up different parts of the nitrogen cycle, including the benefits and threats, can deliver a stronger gravity for better management of these issues. Approaches for evaluating of nitrogen flows will be developed at the level of regional demonstration.